This work investigated the metabolites and their biosynthetic functional hydroxylase genes of the deep-sea sediment metagenomic clone 25D7. 5-Bromoindole was added to the 25D7 clone derived Escherichia coli fermentation broth. The new-generated metabolites and their biosynthetic byproducts were located through LC-MS. in which the isotope peaks of brominated products emerged. https://www.nacrack.com/great-pick-Refurbished-Sawgrass-Virtuoso-SG500-Complete-Sublijet-Sublimation-Printer-Kit-flash-buy/